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KMID : 1157520170130020073
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International Journal of Clinical Preventive Dentistry
2017 Volume.13 No. 2 p.73 ~ p.80
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Reliability of Quantitative Analysis of Bacteria Causing Periodontal Disease by MRT-PCR
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Hwang Kyung-Sook
Cho Ja-Won
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Abstract
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Objective: The purpose of this study was to investigate the oral diseases such as periodontal disease and implantitis infection by quantitative analysis of periodontal disease bacteria in the dentistry field, and to compare the SRT-PCR, which is widely used for prevention purposes.
Methods: Sixty one patients who visited the Dankook University Dental Hospital were selected. were selected. After receiving the consent of the clinical trial, they were gagged for 30 seconds with a syringe and gathered in a 50 ml conical tube. 1 ml was isolated from the collected 20 ml gaggle and DNA was extracted with direct PCR buffer. To compare the sensitivity and specificity of MRT-PCR and SRT-PCR, we used 11 newly-developed primers and probes for the analysis of extracted DNA. At this time, the PCR reaction conditions of MRT-PCR and SRT-PCR were the same as those of pre-denaturation at 95°C for 10 min, denaturation at 95°C for 15 sec, annealing at 63°C for 40 sec.
Results: As a result of quantitative analysis of the 11 species of periodontal disease caused by SRT-PCR and MRT-PCR in TEST I and TEST II studies, 94%-101%, and there was no statistical difference between the two methods.
Conclusion: The accuracy of mutual quantitative analysis of SRT-PCR and MRT-PCR was confirmed by clinical studies, and there was no statistically significant difference. Therefore, it is expected that the dental clinic will become a very useful quantitative test method for screening the causative bacteria of periodontal disease for individual patients in the future.
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KEYWORD
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polymerase chain reaction, periodontal diseases, peri-implantitis
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